Migration type (mt) groupings from Single strand conformation polymorphism (SSCP) analysis of Phytophthora spp. based on largest observed 6-FAM and HEX peaks using GeneScan polymer and an ABI 3100.  Species not listed were found to be uniquea.

 

Groupb

Phytophthora Speciesc

Nd

Lower 95%e CI 6-FAM

Upper 95% CI  6-FAM

Lower 95% CI  HEX

Upper 95% CI  HEX

A

boehmeriae –MT 2

1

7124

7129

7397

7412

A, B

capsici- MT 1

10

7122

7167

7390

7431

B

capsici- MT 2

6

7134

7147

7392

7427

B

capsici -MT 5

1

7133

7161

7333

7393

C

citricola- MT 2

3

7278

7294

7384

7411

C, D

citricola- MT 3

3

7230

7316

7351

7425

D

gonapodyides- MT 1

3

7241

7263

7352

7418

D

inflata- MT 2

2

7252

7255

7344

7359

E

citricola- MT 4

1

7269

7269

7458

7458

E, F

citricola- MT 1

5

7245

7293

7427

7477

F

lateralis- MT 2

1

7273

7279

7469

7474

G

citricola- MT 5

1

7196

7199

7259

7270

G

drechsleri- MT 1

2

7197

7203

7259

7278

H

colocasiae

2

7328

7330

7565

7568

H, I

citrophthora- MT 3

1

7330

7346

7559

7602

I

meadii- MT 3

1

7340

7343

7569

7569

J

boehmeriae -MT 1

1

7360

7365

7586

7598

J

ilicis

1

7364

7379

7571

7593

K

brassicae

4

7380

7400

7502

7537

K

erythroseptica

6

7385

7399

7504

7513

K

cactorum -MT 2

1

7389

7398

7497

7511

L

cryptogea -MT 1

12

7405

7423

7484

7512

L

drechsleri -MT 5

1

7417

7423

7499

7502

L

cambivora -MT 4

1

7413

7419

7495

7498

M

drechsleri -MT 2

2

7393

7401

7532

7543

M

cryptogea - MT

10

7394

7408

7537

7541

M

cryptogea -MT 7

1

7401

7404

7537

7543

N

captiosa

7

7442

7456

7500

7514

N

primulae

2

7448

7459

7500

7544

O

megasperma -MT 1

6

7489

7532

7229

7252

O

cactorum -MT 3

1

7499

7521

7230

7252

P

palmivora -MT 1

8

7504

7515

7716

7738

P

arecae

2

7507

7515

7716

7732

Q

cactorum -MT 1

6

7547

7583

7686

7716

Q

pseudotsugae

4

7550

7587

7684

7721

Q

infestans

1

7567

7570

7705

7705

R

nicotianae -MT 1

14

7543

7575

7806

7843

R

nicotianae -MT 2

4

7567

7603

7807

7860

S

syringae

14

7556

7586

7556

7586

S

tropicalis -MT 2

1

7574

7577

7574

7577

T

asparagi -MT 1

2

7572

7610

7644

7674

T

cryptogea -MT 4

2

7581

7630

7572

7693

T

insolita -MT 2

1

7604

7607

7648

7651

U

melonis

2

7595

7645

7697

7743

U

drechsleri -MT 3

2

7601

7626

7696

7714

U

cryptogea -MT 8

1

7610

7610

7707

7710

V

alni -MT 1

7

7603

7653

7646

7653

V

alni -MT 3

2

7610

7627

7643

7658

W

cambivora -MT 2

3

7626

7657

7704

7726

W, X

cambivora - MT 1

9

7642

7661

7718

7729

W, X

cryptogea - MT 5

2

7634

7658

7691

7737

X

multivesiculata

8

7642

7663

7727

7756

Y

cryptogea -MT 3

2

7633

7644

7244

7250

Y

medicaginis -MT 2

1

7637

7646

7241

7250

Z

gonapodyides -MT 2

2

7627

7675

7658

7690

Z

fragariae

4

7638

7689

7644

7681

Z

cambivora -MT 3

2

7662

7667

7660

7668

AA

inundata

1

7680

7683

7652

7658

AA, BB, CC

humicola

2

7651

7706

7634

7697

BB

asparagi -MT 2

1

7658

7658

7682

7698

CC

insolita -MT 1

1

7680

7686

7660

7666

 

 

a Refer to Table 1 and the supplementary data file in the online e-Xtra for a complete list of Phytophthora species and observed migration types (MT’s) within species.

b Species with the same group letter are indistinguishable based on 95% confidence intervals (CIs) around the means for the largest 6-FAM and HEX peaks.

c Unique MT’s were assigned apriori based on obvious scanline differences, i.e., significantly different means or presence of more than one unique 6-FAM and/or HEX signal, among isolates within species.

d N = the number of samples analyzed per migration type.  Each sample was amplified and subject to SSCP analysis twice.

e Approximate lower and upper 95% CIs for each species or migration type = mean scanline value of largest 6-FAM or HEX peak ± 2* std dev.