Isolate Browser

Isolate ID

 PD_02100 (Phytophthora pinifolia )

Source(s) and alternative IDs

P16100 ( WPC ) 122924 ( CBS ) B, CMW 26668

Host of origin

N/A

Substrate

 

Geographic origin

 Chile

Pathogenic Race

 

Date of Isolation

 Jul. 2007

Mating Type

 Unknown

Characteristics

 

Reference

 

Remarks

 Ex-holotype. Received in 2008 from M. Gryzenhout of Department of Microbiology and Plant Pathology and Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa. Location: Arauco, Llico plantation. Disease of Pinus radiata plantations, chiefly in the Arauco province of Chile . This disease, locally referred to as Daño Foliar del Pino (DFP), is typified by the relatively rapid death of needles and subsequent defoliation of trees. Phytophthora pinifolia sp. nov. associated with a serious needle disease of Pinus radiata in Chile Authors: Durán, A.; Gryzenhout, M.1; Slippers, B.2; Ahumada, R.; Rotella, A.3; Flores, F.3; Wingfield, B. D.2; Wingfield, M. J.1 Source: Plant Pathology, Volume 57, Number 4, August 2008 , pp. 715-727(13) Publisher: Blackwell Publishing Abstract: During the course of the past three years, a new disease of Pinus radiata, referred to as `Daño Foliar del Pino' (DFP) has appeared in the Arauco province of Chile and subsequently spread to other areas. The disease is typified by needle infections, exudation of resin at the bases of the needle brachyblasts and, in younger trees, necrotic lesions in the cambium, which eventually girdle the branches. The disease causes the death of young seedlings and mature trees can also succumb after a few years of successive infection, probably hastened by opportunistic fungi such as Diplodia pinea. Isolations on selective medium for Phytophthora spp. led to the consistent isolation of a Phytophthora sp. from needle tissue. DNA sequence comparisons for the ITS rDNA and cox II gene regions, and morphological observation showed that this oomycete represents a previously undescribed species for which the name Phytophthora pinifolia sp. nov. is provided. This new species is characterized by unbranched sporangiophores, and non-papillate, sub-globose to ovoid sporangia that are occasionally free from the sporangiophore with medium length pedicels. Despite using a number of oospore inducing techniques, oogonia/antheridia were not observed in isolates of P. pinifolia. Pathogenicity trials with P. pinifolia showed that it is pathogenic to P. radiata and causes rapid death of the succulent apical parts of young plants. Phytophthora pinifolia is the first Phytophthora known to be associated with needles and shoots of a Pinus sp. and its aerial habit is well matched with the occurrence and symptoms of DFP in Chile.

Available marker sequences

Chk	Marker	Sequence	Length
	Mitochondrial cox2 locus	PD_02100_cox2	1007 bp
	Internal transcribed spacer region of rDNA	PD_02100_ITS	812 bp
	Mitochondrial NADH Dehydrogenase Subunit 9	PD_02100_nad9	558 bp
	Mitochondrial cox1 locus	PD_02100_cox1	680 bp