Title
Real-time Quantitative PCR: DNA Determination in Isolated Spores of the
Mycorrhizal Fungus Glomus mosseae and Monitoring of Phytophthora infestans and
Phytophthora citricola in their Respective Host Plants
Abstract
Specific primers and dual-labelled fluorogenic probes were designed for polymerase chain reaction (PCR)-based detection of both, mycorrhizal and pathogen DNA. Based on the on!line connection with an auto-mated ABI Prism 7700 sequence detector, amplicon quantification was directly performed during the PCR. The starting copy numbers of target sequences present in each reaction were calculated by comparing the Ct-values of unknown samples to the Ct-values of standards with known amounts of DNA. The Ct-value depends on the
input of starting copies and is defined as that cycle number at which a statistically signicant increase in the reporter fluorescence can first be detected.
Authors
J. BOHM, A. HAHN, R. SCHUBERT, G. BAHNWEG, N. ADLER, J. NECHWATAL, R. OEHLMANN, W. OSWALD
Journal
Journal of Phytopathology, 1999 : 147(0), 409~416
